Background: In routine clinical practice Urinary tract infections are one of the most common bacterial infections.In many hospitals, Urinary tract infection is also the most common nosocomial infection. For formulating antibioticpolicy, study of causative agents and their antimicrobial sensitivity pattern are important factors. As the antibiogramof microorganisms are frequently changing, the present study was done to analyze the recent antibiotic sensitivitypattern of most common Uropathogens E. coli in urinary tract infection in female. Objectives: This study was aimedto evaluate the uropathogens causing UTI in female and antimicrobial susceptibility patterns of E. coli who attended atDiabetic Association Medical College (DAMCH), Faridpur, Bangladesh. Materials and Methods: A cross-sectionaldescriptive type of study was conducted in DAMCH, Faridpur from October 2023 to October 2024. Patients whocame with the symptoms of urinary tract infection were sent for urine culture and disc sensitivity test. The results werecollected and analyzed further. Results: Among 281 urine culture reports 70 (24.91%) cases were culture positivefor urinary pathogens. Post-menopausal women were more affected 41 (58.57%) than women of reproductive ages,22 (31.43%). The Gram-negative bacteria, Escherichia coli was the most common isolates 54 (77.14%) followed byKlebsiella 11 (15.71%), Pseudomonas 4(5.71%) & Proteus 1(1.43%). E.coli was found more sensitive to Amikacin(98.08%), Gentamicin (96.30%), Meropenem (96.30%), Imipenem (96.00%), Tigecycline (95.83%) and Netilmicin(94.23%). On the other hands, most of the Cephalosporin group of drugs, Co-trimoxazole, Amoxiclav and Ciprofloxacinwere the antibiotics had the highest resistance rates. Conclusion: As drug resistance among bacterial pathogens varywith time, regular surveillance and monitoring is necessary for giving updated information to physician for mosteffective empirical treatment of UTIs.