Volume 6

Number 1 January 2022

Evaluation of a Newly Developed Non-Culture Test for Bacteriological Diagnosis of Ventilator-Associated Pneumonia in RMCH

DOI: https://doi.org/10.70357/jdamc.2022.v0601.05

Shohid S1 , Ferdaus F2 , Sinha S3 , Hossain MB4 , Yesmin S5

Abstract

Background: The diagnosis and treatment of pneumonia in patients who are receiving mechanical ventilation remain adifficult challenge.

Objective: To investigate the utility of soluble triggering receptor expressed on myeloid cells1(s TREM-1) levels inEndotracheal aspirate (ETA) samples as early biomarkers for the diagnosis of VAP compared with quantitative culture result ofETA.

Methods: A total of eighty patients with clinically suspected VAP cases were included in this study and were selectedpurposely. ETA was collected and level Wes TREM-1are measured. On the other hand, VAP was diagnosed by the quantitativeculture of ETA.

Results: The concentration of s TREM-1 in ETA did not discriminate VAP positive from VAP negative patients whencompared to quantitative cultures of ETA as the gold standard. A cut-off value of 68.72pg/ml for human s TREM1 in ETAresulted in a sensitivity of 60.81% and specificity of 62.5%.

Conclusion: It may be concluded that the proposed ELISA kit cannot be used as a rapid diagnostic test for the immediatediagnosis of VAP.

Keywords: Ventilator-associated pneumonia (VAP), infection, Non-culture Test


  1. Assistant Professor, Department of Microbiology

    Khulna City Medical College, Khulna

  2. Assistant Professor and Head, Department of Community Medicine

    Khulna City Medical College, Khulna

  3. Associate Professor, Department of Physiology

    Khulna City Medical College, Khulna

  4. Lecturer

    Khulna Medical College, Khulna

  5. Assistant Professor and Head, Department of Pharmacology and Therapeutics

    Khulna City Medical College, Khulna

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Volume 6, Number 1 January 2022
Page: 20-23