The study was conducted during the period of July 2016 to June 2017 in the Department of Pharmacology and Therapeutics with the collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh to determine the profile of antibacterial effect of Crude Ginger Extract (CGE), Ethanolic Ginger Extract (EGE) and standard antibiotic Amikacin against standard strains of Escherichia coli. It was an exploratory study based on laboratory experiment.

The objectives of the study were a) Determination of inhibitory effects of crude ginger extract by incorporation into Nutrientagar media against Escherichia Coli. b) Antibacterial sensitivity testing of ethanolic ginger extract against Escherichia Coli byusing disc diffusion method. c) Determination of minimum inhibitory concentration (MIC) of ethanolic ginger extract againstthe test organism by broth dilution technique. d) Determination of minimum inhibitory concentration (MIC) of antibioticAmikacin against test organism by broth dilution technique and e) Subculture studies of materials from effective CGE, EGEand Amikacin preparations for confirmation of respective results of Experiments I, III and IV.

It was revealed that the growth of Escherichia coli started to be inhibited from 50% CGE incorporated media and even nocomplete inhibition of growth occurred at 100%. In case of Ethanolic Extract in disc diffusion method sensitivity was seenagainst Escherichia coli zone of inhibition was 7 mm at 25 μg/10 μl, 22 mm at 50 μg/10 μl and, 30 mm at 100 μg/10 μlconcentrations respectively. The broth dilution technique was performed to determine the MICs of EGE and Amikacin. TheMIC of EGE was 500 μg/ml against Escherichia coli and the MIC of Amikacin 1 μg/ml against Escherichia coli. Thesubculture study showed the same results with that of previous experiments.

The study confirmed antibacterial effect of ethanolic ginger extract (EGE) against Escherichia coli. The crude ginger extract (CGE) also had its inhibitory effects against the organism studied. The finding highlights the need for further extensive study to detect and isolate the active ingredients present in the Ginger extract responsible for antibacterial effect.